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Be sure to also check accessories needed Notes from our tech department:. Control cells black trace of the main graph in a and c; filled diamonds in the insert were treated only with OGs, as described in Materials and methods.
Extracellular H2O2 accumulation is normalized per g cell fresh weight b-glucan elicitor to their respective receptors in plant transient in our experimental system. When cells, im- cells Stratmann et al. Neomycin sulphate, an cells retained the ability to respond to other stimuli such inhibitor of phospholipase C Legendre et al. The cell refractory state induced by OGs is reversed by the protein kinase inhibitor Ro and prolonged by the protein phosphatase inhibitor okadaic acid A refractory state, i.
Soybean ness of cells towards a second dose of OGs was found to cells were incubated with the following reagents: EGTA lM , increase even more Fig. For assays performed with EGTA, cells were previously washed extensively and resus- protein kinase inhibitors staurosporine and Ro On the other hand, Fig. Soybean cells previously treated with OGs data not shown.
The response to the second treatment is shown Discussion the G-protein-coupled receptor kinase inhibitor Ro In this paper we provide evidence for the involvement of Moore et al. The DP of the OGs determined the relevant dif- inhibitor okadaic acid. The inhibitory effect of okadaic acid on response or H2O2 accumulation. Effect of the phosphatase inhibitor okadaic acid on the Fig.
Effect of the protein kinase inhibitor Ro on the refractory state of soybean cells induced by OGs. Black trace, refractory state of soybean cells induced by OGs.
Arrows indicate the transient induced by the first OG stimulus.
Furthermore, the concentrations of membrane, which go beyond the extracellular interfer- OGs required to induce morphogenetic effects doses ence with the binding of oligosaccharides to their to fold lower than those able to trigger defence receptor Stratmann et al. The intriguing observation of a and no detectable extracellular H2O2 accumulation.
Although TBB is generally ferent cellular responses. What is still not known is believed to be a powerful and selective inhibitor of whether alternative signalling pathways are activated by protein kinase CK2 Espunya et al.
The Several properties, are likely to be active participants in OG- elicitors are well known inducers of cell desensitization. Nevertheless, the lack of Both microbial Binet et al.
The in cells repeatedly subjected to the same elicitor. Aequorin marker of the refractory state of cells. The results of our complementary approach — to re- ; Brini et al. Cervone Dipartimento Fluhr R, Bowler C The role of calcium and activated oxy- di Biologia Vegetale, Roma, Italy for fruitful discussions on the gens as signals for controlling cross-tolerance.